Characterization and Deconstruction of Oligosaccharides in Black Liquor From Deacetylation Process of Corn Stover (2024)

Wang, W., Chen, X., Katahira, R., & Tucker, M. (2019). Characterization and Deconstruction of Oligosaccharides in Black Liquor From Deacetylation Process of Corn Stover. Frontiers in Energy Research, 7(JUN), Article 54. https://doi.org/10.3389/fenrg.2019.00054, https://doi.org/10.3389/fenrg.2019.00054

Wang, Wei ; Chen, Xiaowen ; Katahira, Rui et al. / Characterization and Deconstruction of Oligosaccharides in Black Liquor From Deacetylation Process of Corn Stover. In: Frontiers in Energy Research. 2019 ; Vol. 7, No. JUN.

@article{24912a76ec1c4a58bd9fb21631f43a8b,

title = "Characterization and Deconstruction of Oligosaccharides in Black Liquor From Deacetylation Process of Corn Stover",

abstract = "The National Renewable Energy Laboratory (NREL) developed low severity Deacetylation and Mechanical Refining (DMR) process under atmospheric pressure, generates highly fermentable, low toxicity sugar syrups and highly reactive lignin streams. The dilute alkali deacetylation step saponifies acetyl groups from the hemicellulosic fraction of biomass into a black liquor waste stream that also contains solubilized acetic, ferulic, p-coumaric acids, lignin-carbohydrate complexes (LCCs), oligosaccharides, and solubilized lignin. Valorization of the soluble components in the deacetylation black liquor waste stream requires characterization of the black liquor. Our analyses show that glucan, xylan, and lignin were three main components in black liquor and the oligosaccharides in the black liquor were mainly dimers and trimers with arabinofuranose branch groups mainly on the xylooligomers. GPC chromatograms showed that the black liquor also contained oligomeric lignin moieties with molecular weights ranging between 1,800 and ~10,000 Da. Enzymatic saccharification of the black liquor was conducted based on the compositional and structural information. Incorporation of commercial cellulase and hemicellulase enzyme preparations with various accessary enzymes showed up to 57% hydrolysis of the xylooligosaccharides in the black liquor to monomeric sugars, which could be used to improve biorefinery carbon utilization in biological fermentations to improve end product fuel or bioproduct volumes per metric ton of biomass processed. This is so far the first study about characterizing DMR black liquor and exploring breaking down the oligosaccharides in black liquor for potential use.",

keywords = "Black liquor, Characterization, Deacetylation, Enzymatic saccharification, Xylooligosaccharides",

author = "Wei Wang and Xiaowen Chen and Rui Katahira and Melvin Tucker",

note = "Publisher Copyright: {\textcopyright} 2019 Wang, Chen, Katahira and Tucker.",

year = "2019",

doi = "10.3389/fenrg.2019.00054",

language = "American English",

volume = "7",

journal = "Frontiers in Energy Research",

issn = "2296-598X",

publisher = "Frontiers Media S.A.",

number = "JUN",

}

Wang, W, Chen, X, Katahira, R & Tucker, M 2019, 'Characterization and Deconstruction of Oligosaccharides in Black Liquor From Deacetylation Process of Corn Stover', Frontiers in Energy Research, vol. 7, no. JUN, 54. https://doi.org/10.3389/fenrg.2019.00054, https://doi.org/10.3389/fenrg.2019.00054

Characterization and Deconstruction of Oligosaccharides in Black Liquor From Deacetylation Process of Corn Stover. / Wang, Wei; Chen, Xiaowen; Katahira, Rui et al.
In: Frontiers in Energy Research, Vol. 7, No. JUN, 54, 2019.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Characterization and Deconstruction of Oligosaccharides in Black Liquor From Deacetylation Process of Corn Stover

AU - Wang, Wei

AU - Chen, Xiaowen

AU - Katahira, Rui

AU - Tucker, Melvin

N1 - Publisher Copyright:© 2019 Wang, Chen, Katahira and Tucker.

PY - 2019

Y1 - 2019

N2 - The National Renewable Energy Laboratory (NREL) developed low severity Deacetylation and Mechanical Refining (DMR) process under atmospheric pressure, generates highly fermentable, low toxicity sugar syrups and highly reactive lignin streams. The dilute alkali deacetylation step saponifies acetyl groups from the hemicellulosic fraction of biomass into a black liquor waste stream that also contains solubilized acetic, ferulic, p-coumaric acids, lignin-carbohydrate complexes (LCCs), oligosaccharides, and solubilized lignin. Valorization of the soluble components in the deacetylation black liquor waste stream requires characterization of the black liquor. Our analyses show that glucan, xylan, and lignin were three main components in black liquor and the oligosaccharides in the black liquor were mainly dimers and trimers with arabinofuranose branch groups mainly on the xylooligomers. GPC chromatograms showed that the black liquor also contained oligomeric lignin moieties with molecular weights ranging between 1,800 and ~10,000 Da. Enzymatic saccharification of the black liquor was conducted based on the compositional and structural information. Incorporation of commercial cellulase and hemicellulase enzyme preparations with various accessary enzymes showed up to 57% hydrolysis of the xylooligosaccharides in the black liquor to monomeric sugars, which could be used to improve biorefinery carbon utilization in biological fermentations to improve end product fuel or bioproduct volumes per metric ton of biomass processed. This is so far the first study about characterizing DMR black liquor and exploring breaking down the oligosaccharides in black liquor for potential use.

AB - The National Renewable Energy Laboratory (NREL) developed low severity Deacetylation and Mechanical Refining (DMR) process under atmospheric pressure, generates highly fermentable, low toxicity sugar syrups and highly reactive lignin streams. The dilute alkali deacetylation step saponifies acetyl groups from the hemicellulosic fraction of biomass into a black liquor waste stream that also contains solubilized acetic, ferulic, p-coumaric acids, lignin-carbohydrate complexes (LCCs), oligosaccharides, and solubilized lignin. Valorization of the soluble components in the deacetylation black liquor waste stream requires characterization of the black liquor. Our analyses show that glucan, xylan, and lignin were three main components in black liquor and the oligosaccharides in the black liquor were mainly dimers and trimers with arabinofuranose branch groups mainly on the xylooligomers. GPC chromatograms showed that the black liquor also contained oligomeric lignin moieties with molecular weights ranging between 1,800 and ~10,000 Da. Enzymatic saccharification of the black liquor was conducted based on the compositional and structural information. Incorporation of commercial cellulase and hemicellulase enzyme preparations with various accessary enzymes showed up to 57% hydrolysis of the xylooligosaccharides in the black liquor to monomeric sugars, which could be used to improve biorefinery carbon utilization in biological fermentations to improve end product fuel or bioproduct volumes per metric ton of biomass processed. This is so far the first study about characterizing DMR black liquor and exploring breaking down the oligosaccharides in black liquor for potential use.

KW - Black liquor

KW - Characterization

KW - Deacetylation

KW - Enzymatic saccharification

KW - Xylooligosaccharides

UR - http://www.scopus.com/inward/record.url?scp=85069056587&partnerID=8YFLogxK

U2 - 10.3389/fenrg.2019.00054

DO - 10.3389/fenrg.2019.00054

M3 - Article

AN - SCOPUS:85069056587

SN - 2296-598X

VL - 7

JO - Frontiers in Energy Research

JF - Frontiers in Energy Research

IS - JUN

M1 - 54

ER -

Wang W, Chen X, Katahira R, Tucker M. Characterization and Deconstruction of Oligosaccharides in Black Liquor From Deacetylation Process of Corn Stover. Frontiers in Energy Research. 2019;7(JUN):54. doi: 10.3389/fenrg.2019.00054, 10.3389/fenrg.2019.00054